October 3, 2018

Assay System:

  • Blood plasma from desired species.
  • Stability determined by measuring percent remaining of test compound at multiple time points during incubation in plasma.

Assay Conditions:

Test Compound Concentration10 µM
Percent Organic Solvent in Incubation0.5% acetonitrile
Incubation Time0, 15, 30, 60, and 90 minutes
pH7.4
Temperature37 °C
Positive ControlPropantheline and/or Enalapril
AnalysisLC-MS (LC-MS/MS if necessary)
Number of Repeats2

Deliverable: Half-life of test compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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