October 3, 2018

Assay System:

  • Brain tissue (other tissues and biological fractions available upon request).
  • Protein binding of test compound measured using Rapid Equilibrium Dialysis (RED) device.

Assay Conditions:

Test Compound Concentration1 µM
Percent Organic Solvent in Incubation0.5% acetonitrile
Incubation Time4 hours
pH7.4, adjusted prior to incubation
Temperature37 °C
Positive ControlPropranolol
AnalysisLC-MS/MS
Number of Repeats3
Brain HomogenatePrepared in 4 volumes of phosphate-buffered saline (PBS)

Deliverable: Percent bound of test compound and positive control compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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