October 3, 2018

Assay System:

  • Lipid-infused artificial membrane.
  • Passive transport of test compound measured through permeability across a phospholipid membrane.

Assay Conditions:

Test Compound Concentration100 µM
Percent Organic Solvent in Incubation1% DMSO
Incubation Time5 hours
pH7.4
Temperature20 °C
Positive ControlVerapamil
AnalysisUV Absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats2

Deliverable: Passive permeability (Pe), mass retention (R)

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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