October 3, 2018

Assay System:

  • Mammalian liver cytosol or major human recombinant N-acetyltransferases.
  • Formation of N-acetylated metabolites of test compound measured upon incubation with liver cytosol or recombinant N-acetyltransferases.
  • Position of acetylation in the test compound can be elucidated upon request.

Assay Conditions: 

Test Compound Concentration5 µM
Percent Organic Solvent in Incubation1% acetonitrile
Incubation Time30 minutes
pH7.4
Temperature37 °C
Positive ControlSulfamethazine
AnalysisLC-MS/MS
Number of Repeats3

Deliverable: Formation of N-acetylated metabolites (% of parent) and determination of acetylation site (optional).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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