October 3, 2018
- Mammalian liver cytosol or major human recombinant N-acetyltransferases.
- Formation of N-acetylated metabolites of test compound measured upon incubation with liver cytosol or recombinant N-acetyltransferases.
- Position of acetylation in the test compound can be elucidated upon request.
|Test Compound Concentration||5 µM|
|Percent Organic Solvent in Incubation||1% acetonitrile|
|Incubation Time||30 minutes|
|Number of Repeats||3|
Deliverable: Formation of N-acetylated metabolites (% of parent) and determination of acetylation site (optional).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.