October 3, 2018

Minimum Inhibitory Concentrations (MICs) are determined using a modified CLSI microbroth dilution assay protocol (document M7), including clinical isolates representing both current and developing antibiotic resistance in a 384-well microplate format which can accommodate 8 reference antibiotics or 8 test compounds, in triplicate, with a 12-point, 1:2 dilution series (plus appropriate controls). The CLSI guidelines result in a cutoff determination for the MIC.

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