October 3, 2018
- Mammalian liver microsomes.
- Metabolic clearance determined by measuring percent remaining of test compound at a single time point after incubation with liver microsomes.
|Test Compound Concentration||1 µM|
|Percent Organic Solvent in Incubation||0.5% acetonitrile|
|Incubation Time||0 and 30 minutes|
|Analysis||LC-MS (LC-MS/MS if necessary)|
|Number of Repeats||2|
Deliverable: Percent remaining of test compound after 30 min incubation with liver microsomes. Incubation time can be adjusted as needed, upon request.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.