October 3, 2018

Assay System:

  • Mammalian liver microsomes.
  • Metabolic clearance determined by measuring percent remaining of test compound at a single time point after incubation with liver microsomes.

Assay Conditions:

Test Compound Concentration1 µM
Percent Organic Solvent in Incubation0.5% acetonitrile
Incubation Time0 and 30 minutes
pH7.4
Temperature37 °C
Positive ControlTestosterone
AnalysisLC-MS (LC-MS/MS if necessary)
Number of Repeats2

Deliverable: Percent remaining of test compound after 30 min incubation with liver microsomes. Incubation time can be adjusted as needed, upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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