October 3, 2018

Assay System:

  • Liver microsomes, S9 fractions, or hepatocytes
  • Samples for analysis are generated in the course of in vitro clearance (metabolic stability) studies
  • Identification of metabolites of test compound generated in Phase I and/or Phase II metabolic reactions using a High Resolution LC-MS instrument
  • Determination of tentative structures of metabolites using advanced LC-MS/MS analysis

Assay Conditions:

Test Compound Concentration1 µM
Incubation Time0.5-4 hours
pH7.4
Temperature37 °C
AnalysisLC-MS/MS
Number of Repeats2

Deliverable:

  • Tier 1 analysis: A list of identified metabolites, including corresponding molecular masses and relative abundance of metabolites estimated from integration of peak areas in LC/MS/MS chromatograms.
  • Note: For Tier 2 analysis (includes Tier 1 analysis results, plus MS/MS spectra of identified metabolites, suggested metabolite structures, and possible metabolic pathways of test compound) please select Standard Metabolite Profiling and Identification.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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