October 3, 2018

Assay System:

  • Liver S9 fractions
  • LC-MS/MS detection of reactive metabolites of test compound using glutathione, KCN, or methoxyl amine as trapping reagents

Assay Conditions:

Test Compound Concentration50 µM
Incubation Time3 hours
pH7.4
Temperature37 °C
Positive ControlClozapine (for glutathione and KCN trapping);
MTEP (3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine hydrochloride) (for methoxyl amine trapping)
AnalysisLC-MS/MS
Number of Repeats2

Deliverable: Molecular weights and relative abundance of detected conjugates of test compound with glutathione, KCN, or methoxyl amine. Tentative structures of conjugates determined using MS/MS analysis are available upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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