October 3, 2018

Assay System:

  • Liver microsomes
  • LC-MS/MS detection of reactive metabolites of test compound using glutathione or KCN as trapping reagents

Assay Conditions:

Test Compound Concentration50 µM
Incubation Time3 hours
pH7.4
Temperature37 °C
Positive ControlClozapine
AnalysisLC-MS/MS
Number of Repeats2

Deliverable: Molecular weights and relative abundance of detected conjugates of test compound with glutathione or KCN. Tentative structures of conjugates determined using MS/MS analysis are available upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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