October 3, 2018

Assay System: 

  • Individually expressed recombinant human CYPs (rhCYPs).
  • Human CYP isoforms responsible for metabolism determined by measuring disappearance of test compound in incubations with individual rhCYPs.

Assay Conditions: 

Test Compound Concentration1 µM
Incubation Time30 minutes
pH7.4
Temperature37°C
AnalysisLC-MS/MS
Number of Repeats2

Study Report: Percent of test compound remaining after incubation with individual rhCYPs.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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