October 3, 2018

Assay System:

  • Human liver microsomes.
  • Human CYP isoforms responsible for metabolism of test compound determined by measuring metabolism suppression in the presence of CYP isoform-selective inhibitors.

Assay Conditions: 

Test Compound Concentration1 µM
Incubation Time30 minutes
pH7.4
Temperature37 °C
Positive ControlCYP isoform-selective probe substrates
AnalysisLC-MS/MS
Number of Repeats2

Study Report: Percent of metabolism of test compound suppressed by CYP isoform-selective inhibitors.

Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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