October 3, 2018

Assay System:

  • Standard test systems include Phosphate Buffered Saline (PBS) pH 7.4, Simulated Gastric Fluid (SGF), and Simulated Intestinal Fluid (SIF).
  • Additional test systems available upon request.
  • Stability determined by measuring percent remaining of test compound at multiple time points during incubation in selected media.

Assay Conditions:

Test Compound Concentration10 µM
Percent Organic Solvent in Incubation1% DMSO
Incubation Time (PBS)0, 2, 4, 6, 8, 24, 48, 72 hours
Incubation Time (SGF, SIF)0, 15, 30, 60, and 90 minutes
pH7.4 (PBS), 1.2 (SGF), 5.0 (fed-state SIF), 6.5 (fasted-state SIF)
Positive ControlVerapamil and Tamoxifen
AnalysisLC-MS (LC-MS/MS if necessary)
Number of Repeats2

Half-life of test compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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