October 3, 2018
- Standard test systems include Phosphate Buffered Saline (PBS) pH 7.4, Simulated Gastric Fluid (SGF), and Simulated Intestinal Fluid (SIF).
- Additional test systems available upon request.
- Stability determined by measuring percent remaining of test compound at multiple time points during incubation in selected media.
|Test Compound Concentration||10 µM|
|Percent Organic Solvent in Incubation||1% DMSO|
|Incubation Time (PBS)||0, 2, 4, 6, 8, 24, 48, 72 hours|
|Incubation Time (SGF, SIF)||0, 15, 30, 60, and 90 minutes|
|pH||7.4 (PBS), 1.2 (SGF), 5.0 (fed-state SIF), 6.5 (fasted-state SIF)|
|Positive Control||Verapamil and Tamoxifen|
|Analysis||LC-MS (LC-MS/MS if necessary)|
|Number of Repeats||2|
Deliverable: Half-life of test compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.