October 3, 2018

Assay System:

  • Fresh blood from desired animal species.
  • Partitioning of test compound measured between red blood cells (RBC) and plasma.

Assay Conditions:

Test Compound Concentration10 µM
Percent Organic Solvent in Incubation1% acetonitrile
Incubation Time60 minutes
pHadjusted by 2-fold dilution of blood with PBS buffer prior to incubation
Temperature37 °C
Positive ControlChlorthalidone
AnalysisLC-MS/MS
Number of Repeats3

Deliverable: RBC-to-Plasma ratio (KRBC/PL).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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