October 3, 2018

Assay System:

  • Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
  • Solubility measured by incubating test compound in PBS for 24 h, followed by determination of dissolved concentration against a calibration curve.

Assay Conditions:

Test Compound Concentration100 µM
Percent Organic Solvent in IncubationNo organic solvent added
Incubation Time24 hours
pH7.4 or as requested
TemperatureAmbient
Positive ControlVerapamil and Tamoxifen
AnalysisUV/VIS Absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats2

Deliverable: Solubility of test compound (µM).

Test Compound Requirement: 1-2 mg dry powder.

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