October 3, 2018

Assay System:

  • Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
  • Kinetic aqueous solubility measured by incubating test compound in PBS for 1 h, followed by determination of dissolved concentration against a calibration curve.

Assay Conditions:

Test Compound Concentration100 µM
Percent Organic Solvent in Incubation1% DMSO
Incubation Time1 hour
pH7.4 or as requested
Positive ControlVerapamil and Tamoxifen
AnalysisUV Absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats2

Deliverable: Solubility of test compound (µM).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

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