October 3, 2018
- Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
- Kinetic aqueous solubility measured by incubating test compound in PBS for 1 h, followed by determination of dissolved concentration against a calibration curve.
|Test Compound Concentration||100 µM|
|Percent Organic Solvent in Incubation||1% DMSO|
|Incubation Time||1 hour|
|pH||7.4 or as requested|
|Positive Control||Verapamil and Tamoxifen|
|Analysis||UV Absorbance or LC-MS (LC-MS/MS if necessary)|
|Number of Repeats||2|
Deliverable: Solubility of test compound (µM).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.