October 3, 2018

  • Characterization of Antibody-Drug Conjugate (ADC) using Sciex TripleTOF 6600 System
  • Determination of drug-to-antibody ratio (DAR)
  • Analysis of both intact and reduced ADC
  • For reduced ADC analysis, ADC is incubated with DTT at 37 °C for 30 minutes

Deliverables:

  • Drug-to-antibody ratio (DAR)
  • m/z profile of ADC
  • Reconstructed mass of the components of ADC

Test Compound Requirement:

  • 100 µg lyophilized ADC  or 50 µL of 2 mg/mL solution in PBS for intact protein analysis
  • 100 µg lyophilized ADC  or 50 µL of 2 mg/mL solution in TBS (pH 8-8.5) for reduced ADC analysis

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